For protein synthesis, the amino acids are recognized and carried by:
- (a)mRNA
- (b)snRNA
- (c)miRNA
- (d)tRNA
Correct — D, tRNA. Transfer RNA is the adaptor that lets a language of nucleotides specify a language of amino acids. Each tRNA is a small folded molecule, around seventy-six nucleotides long, with two working ends. At one end sits the anticodon, a triplet that base-pairs with a matching codon on the messenger RNA held in the ribosome. At the other end, the CCA tail, the amino acid is attached. The matching of the right amino acid to the right tRNA is done beforehand by an aminoacyl-tRNA synthetase, one for each amino acid, and that enzyme is where the genetic code is physically enforced. So the tRNA recognises its amino acid through its synthetase, carries it to the ribosome, and hands it over only where its anticodon fits — recognition and transport in one molecule, which is exactly what the question describes.
- (a)mRNA — Messenger RNA carries the instruction, not the cargo. It is the transcript of a gene, read three bases at a time, and it never binds an amino acid.
- (b)snRNA — Small nuclear RNA works before translation begins. Combined with proteins into snRNPs it forms the spliceosome, which cuts introns out of the primary transcript inside the nucleus.
- (c)miRNA — MicroRNA is a regulator. Around twenty-two nucleotides long, it pairs with target messenger RNAs and either blocks their translation or triggers their degradation, so it reduces protein output rather than supplying its raw material.
Protein synthesis runs in two stages. Transcription copies a gene into messenger RNA in the nucleus; translation reads that message on a ribosome in the cytoplasm and builds the polypeptide. Four classes of RNA appear in the process. Messenger RNA carries the code, ribosomal RNA forms the catalytic core of the ribosome itself, transfer RNA delivers amino acids, and a family of small RNAs — snRNA, snoRNA, miRNA, siRNA — handles processing and regulation. The genetic code has sixty-four codons for twenty amino acids, so most amino acids answer to more than one codon.
Option lists in this topic are built from the RNA alphabet soup, and the reliable way through it is to hold one function per name rather than to guess from the letters. A useful check is the direction of travel: mRNA moves information out of the nucleus, tRNA moves amino acids into the ribosome, rRNA does not move at all. Note also that the ribosome's peptide-bond-forming site is made of RNA, not protein, which is why the ribosome is called a ribozyme — a detail that shows up as a stand-alone question often enough to be worth carrying.
- A transfer RNA is about seventy-six nucleotides long and folds into a cloverleaf that stacks into an L shape.
- The anticodon triplet pairs with the messenger RNA codon; the amino acid is carried on the 3' CCA end.
- Aminoacyl-tRNA synthetases charge each tRNA with its own amino acid, and there is one such enzyme per amino acid.
- Sixty-four codons cover twenty amino acids plus a start and three stop signals, so the code is degenerate but unambiguous.
- The peptide bond is formed by ribosomal RNA, which makes the ribosome a ribozyme.
- DNA gene is transcribed into messenger RNA in the nucleus
- Messenger RNA moves to the cytoplasm and binds a ribosome
- A synthetase charges each transfer RNA with its own amino acid
- The transfer RNA anticodon pairs with the messenger RNA codon and delivers that amino acid
- Ribosomal RNA joins the amino acids into a growing polypeptide chain
Information flows along the messenger; the amino acids arrive on the transfer RNA.
- Treating mRNA as the carrier of amino acids because it is the molecule the question mentions most often elsewhere.
- Confusing the anticodon on the tRNA with the codon on the mRNA when a question asks which sits where.
- Assuming every RNA in the list is involved in translation; snRNA and miRNA act before and around it.
Asked as a one-line functional identification, with the four RNA classes offered as options and the whole mark resting on knowing which one is the adaptor.
Which of the following cell organelles play the most significant role in protein synthesis?
- (a) Lysosome and Centrosome
- (b) Endoplasmic reticulum and Ribosome
- (c) Golgi apparatus and Mitochondria
- (d) Lysosome and Mitochondria
Answer(b) Endoplasmic reticulum and Ribosome
The same process asked at the level of the compartment rather than the molecule. Prelims wanted the site of translation; CAPF wants the courier that arrives there with the amino acid.
In the context of the developments in Bioinformatics, the term 'transcriptome', sometimes seen in the news, refers to
- (a) a range of enzymes used in genome editing
- (b) the full range of mRNA molecules expressed by an organism
- (c) the description of the mechanism of gene expression
- (d) a mechanism of genetic mutations taking place in cells
Answer(b) the full range of mRNA molecules expressed by an organism
A check on the other half of the pair. If you can say what a transcriptome is made of, you already know that messenger RNA is the message and cannot also be the amino-acid carrier.
Which one of the following is the correct combination of organelles and their functions?
- (a) Mitochondria – Respiration; Chloroplast – Photosynthesis; Ribosome – Protein Synthesis; Rough endoplasmic reticulum – Transport of proteins
- (b) Mitochondria – Respiration; Chloroplast – Photosynthesis; Ribosome – Transport of proteins; Rough endoplasmic reticulum – Protein synthesis
- (c) Mitochondria – Respiration; Chloroplast – Protein synthesis; Ribosome – Photosynthesis; Rough endoplasmic reticulum – Transport of proteins
- (d) Mitochondria – Photosynthesis; Chloroplast – Respiration; Ribosome – Protein synthesis; Rough endoplasmic reticulum – Transport of proteins
Answer(a) Mitochondria – Respiration; Chloroplast – Photosynthesis; Ribosome – Protein Synthesis; Rough endoplasmic reticulum – Transport of proteins
Sorted at the level of the whole cell. It fixes the ribosome as the place where the polypeptide is assembled, which is the destination the transfer RNA is heading for in this item.
- practice — not a real PYQ
The enzyme that attaches an amino acid to its corresponding transfer RNA is
- (a)RNA polymerase
- (b)aminoacyl-tRNA synthetase
- (c)reverse transcriptase
- (d)DNA ligase
Answer(b) aminoacyl-tRNA synthetase — there is one for each amino acid, and its accuracy is what keeps translation faithful.
- practice — not a real PYQ
Which one of the following RNAs forms the catalytic core of the ribosome?
- (a)mRNA
- (b)tRNA
- (c)rRNA
- (d)miRNA
Answer(c) rRNA — ribosomal RNA catalyses peptide-bond formation, which is why the ribosome is described as a ribozyme.